RBS

Part:BBa_K3352002:Design

Designed by: Joyce Ting   Group: iGEM20_TAS_Taipei   (2020-10-18)


Extended RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Utilizing both a T7 promoter, T7 terminator, and an extended UTR sequence around the RBS and before the terminator, we would maximize the protein expression for our enzymes.


Source

pET vectors include the T7 bacteriophage gene 10, which promotes high-level transcription and translation.

References